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PromoCell osteoblast growth medium gm

Glucocorticoids impair <t>osteoblast</t> function and collagen expression in vivo. A) H&E staining of mouse bone after 60 days without (Veh) or with the GC, prednisolone (Psl); Scale bar: 1000 µm. B) Representative images for Alkaline phosphatase (ALP) staining of mouse bones; Scale bar, 400 µm; Magnification: Scale bar, 100 µm. C) Quantitative analysis of the ALP‐positive surface intensity in bone tissue sections for n = 5 mice. D) Representative images for Verhoeff van Geison staining showing general elastin‐ (black) and collagen‐specific proteins (red) of mouse bones; Scale bar, 400 µm; Magnification: Scale bar: 100 µm. E) Semi‐quantitative analysis of the collagen fibers‐positive surface intensity in bone tissue sections; n = 5 mice. F) Representative image of collagen type I alpha I (COL1A1) staining of bone sections. Yellow arrows indicate COL1A1 protein expression (red). Green diamonds indicate the interior of cortical bone, near the bone marrow. Scale bar, 50 µm. G) Graph showing the COL1A1 protein expression levels (artificial units; A.U.) in control and Psl‐treated bone tissues. H) Metabolomics KEGG visualization of in vitro osteo‐spheroids treated without (Veh) and with Psl at 100 µm for 7 d. I,J) Analysis of collagen‐related putative metabolites after significance analysis using Metaboanalyst only reported fold‐changes with p‐value < 0.05. Data are expressed as mean ± SD. N = 5, n = 3; significant differences: * p‐value < 0.05.

Osteoblast Growth Medium Gm, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/osteoblast+growth+medium+gm/pmc13015773-138-7-11?v=PromoCell
Average 95 stars, based on 153 article reviews

osteoblast growth medium gm - by Bioz Stars, 2026-08

95/100 stars

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1) Product Images from "Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation"

Article Title: Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation

Journal: Advanced Healthcare Materials

doi: 10.1002/adhm.202502606

Glucocorticoids impair osteoblast function and collagen expression in vivo. A) H&E staining of mouse bone after 60 days without (Veh) or with the GC, prednisolone (Psl); Scale bar: 1000 µm. B) Representative images for Alkaline phosphatase (ALP) staining of mouse bones; Scale bar, 400 µm; Magnification: Scale bar, 100 µm. C) Quantitative analysis of the ALP‐positive surface intensity in bone tissue sections for n = 5 mice. D) Representative images for Verhoeff van Geison staining showing general elastin‐ (black) and collagen‐specific proteins (red) of mouse bones; Scale bar, 400 µm; Magnification: Scale bar: 100 µm. E) Semi‐quantitative analysis of the collagen fibers‐positive surface intensity in bone tissue sections; n = 5 mice. F) Representative image of collagen type I alpha I (COL1A1) staining of bone sections. Yellow arrows indicate COL1A1 protein expression (red). Green diamonds indicate the interior of cortical bone, near the bone marrow. Scale bar, 50 µm. G) Graph showing the COL1A1 protein expression levels (artificial units; A.U.) in control and Psl‐treated bone tissues. H) Metabolomics KEGG visualization of in vitro osteo‐spheroids treated without (Veh) and with Psl at 100 µm for 7 d. I,J) Analysis of collagen‐related putative metabolites after significance analysis using Metaboanalyst only reported fold‐changes with p‐value < 0.05. Data are expressed as mean ± SD. N = 5, n = 3; significant differences: * p‐value < 0.05.

Figure Legend Snippet: Glucocorticoids impair osteoblast function and collagen expression in vivo. A) H&E staining of mouse bone after 60 days without (Veh) or with the GC, prednisolone (Psl); Scale bar: 1000 µm. B) Representative images for Alkaline phosphatase (ALP) staining of mouse bones; Scale bar, 400 µm; Magnification: Scale bar, 100 µm. C) Quantitative analysis of the ALP‐positive surface intensity in bone tissue sections for n = 5 mice. D) Representative images for Verhoeff van Geison staining showing general elastin‐ (black) and collagen‐specific proteins (red) of mouse bones; Scale bar, 400 µm; Magnification: Scale bar: 100 µm. E) Semi‐quantitative analysis of the collagen fibers‐positive surface intensity in bone tissue sections; n = 5 mice. F) Representative image of collagen type I alpha I (COL1A1) staining of bone sections. Yellow arrows indicate COL1A1 protein expression (red). Green diamonds indicate the interior of cortical bone, near the bone marrow. Scale bar, 50 µm. G) Graph showing the COL1A1 protein expression levels (artificial units; A.U.) in control and Psl‐treated bone tissues. H) Metabolomics KEGG visualization of in vitro osteo‐spheroids treated without (Veh) and with Psl at 100 µm for 7 d. I,J) Analysis of collagen‐related putative metabolites after significance analysis using Metaboanalyst only reported fold‐changes with p‐value < 0.05. Data are expressed as mean ± SD. N = 5, n = 3; significant differences: * p‐value < 0.05.

Techniques Used: Expressing, In Vivo, Staining, Control, In Vitro

AA enhances COL1A1 biological activity and restores osteoblast function and COL1A1 regulation in the presence of Psl. A) ALP staining (red) of osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with (+AA), and in the Psl presence (+Psl) or absence (–Psl); Scale bar: 300 µm. B) Quantification of Mean Intensity of ALP (A.U.). C) Alzarin Red (AR) activity assay of osteo‐spheroids after 7 d with or without additional AA. Scale bar, 400 µm. D) Graph showing quantification of Mean Intensity of AR (A.U.). E) OsteoImaging mineralization assay quantifications of Mean Intensity (A.U.) of osteo‐spheroids incubated with or without additional AA for 7 d. F) Gene expression analysis of osteogenic markers ( BGLAP, DMP1, DLX3, RUNX2 ) of osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with (+AA), and in the Psl presence (+Psl) or absence (–Psl); Heatmap demonstrates the ∆CT averages. G) Gene expression analysis of COL1A1‐related genes ( PLOD1, PLOD3, DLX3, P3H1, P3H2, P3H3, LOX, SVCT2, COL1A2, COL22A1, IBSP, P4HA2, P4HA3, IFITM5 ) in osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with or without ascorbic acid (AA), and in the presence (+Psl) or absence (–Psl) of prednisolone (Psl); Heatmap demonstrates the ∆CT averages H) Fold change of top collagen‐related putative metabolites elevated in the presence of endogenous ascorbic acid (+AA) within the 3D matrix of osteo‐spheroids after 7 d of Psl (+Psl) treatment, based on untargeted metabolomics analysis; fold change threshold > 2 with significance; FDR p‐value < 0.05. The data are expressed as mean ± SD; N = 3, n = 3; significant differences: * * p‐value < 0.01; *** p‐value < 0.005 ; **** p‐value < 0.0001.

Figure Legend Snippet: AA enhances COL1A1 biological activity and restores osteoblast function and COL1A1 regulation in the presence of Psl. A) ALP staining (red) of osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with (+AA), and in the Psl presence (+Psl) or absence (–Psl); Scale bar: 300 µm. B) Quantification of Mean Intensity of ALP (A.U.). C) Alzarin Red (AR) activity assay of osteo‐spheroids after 7 d with or without additional AA. Scale bar, 400 µm. D) Graph showing quantification of Mean Intensity of AR (A.U.). E) OsteoImaging mineralization assay quantifications of Mean Intensity (A.U.) of osteo‐spheroids incubated with or without additional AA for 7 d. F) Gene expression analysis of osteogenic markers ( BGLAP, DMP1, DLX3, RUNX2 ) of osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with (+AA), and in the Psl presence (+Psl) or absence (–Psl); Heatmap demonstrates the ∆CT averages. G) Gene expression analysis of COL1A1‐related genes ( PLOD1, PLOD3, DLX3, P3H1, P3H2, P3H3, LOX, SVCT2, COL1A2, COL22A1, IBSP, P4HA2, P4HA3, IFITM5 ) in osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with or without ascorbic acid (AA), and in the presence (+Psl) or absence (–Psl) of prednisolone (Psl); Heatmap demonstrates the ∆CT averages H) Fold change of top collagen‐related putative metabolites elevated in the presence of endogenous ascorbic acid (+AA) within the 3D matrix of osteo‐spheroids after 7 d of Psl (+Psl) treatment, based on untargeted metabolomics analysis; fold change threshold > 2 with significance; FDR p‐value < 0.05. The data are expressed as mean ± SD; N = 3, n = 3; significant differences: * * p‐value < 0.01; *** p‐value < 0.005 ; **** p‐value < 0.0001.

Techniques Used: Activity Assay, Staining, Cell Culture, Mineralization Assay, Incubation, Gene Expression

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Journal: Advanced Healthcare Materials

Article Title: Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation

doi: 10.1002/adhm.202502606

Figure Lengend Snippet: Glucocorticoids impair osteoblast function and collagen expression in vivo. A) H&E staining of mouse bone after 60 days without (Veh) or with the GC, prednisolone (Psl); Scale bar: 1000 µm. B) Representative images for Alkaline phosphatase (ALP) staining of mouse bones; Scale bar, 400 µm; Magnification: Scale bar, 100 µm. C) Quantitative analysis of the ALP‐positive surface intensity in bone tissue sections for n = 5 mice. D) Representative images for Verhoeff van Geison staining showing general elastin‐ (black) and collagen‐specific proteins (red) of mouse bones; Scale bar, 400 µm; Magnification: Scale bar: 100 µm. E) Semi‐quantitative analysis of the collagen fibers‐positive surface intensity in bone tissue sections; n = 5 mice. F) Representative image of collagen type I alpha I (COL1A1) staining of bone sections. Yellow arrows indicate COL1A1 protein expression (red). Green diamonds indicate the interior of cortical bone, near the bone marrow. Scale bar, 50 µm. G) Graph showing the COL1A1 protein expression levels (artificial units; A.U.) in control and Psl‐treated bone tissues. H) Metabolomics KEGG visualization of in vitro osteo‐spheroids treated without (Veh) and with Psl at 100 µm for 7 d. I,J) Analysis of collagen‐related putative metabolites after significance analysis using Metaboanalyst only reported fold‐changes with p‐value < 0.05. Data are expressed as mean ± SD. N = 5, n = 3; significant differences: * p‐value < 0.05.

Article Snippet: Human osteoblasts (HOBs) (PromoCell) were cultured in osteoblast growth medium (GM) (PromoCell) and differentiated in osteoblast mineralization medium (MM) (PromoCell).

Techniques: Expressing, In Vivo, Staining, Control, In Vitro

Journal: Advanced Healthcare Materials

Article Title: Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation

doi: 10.1002/adhm.202502606

Figure Lengend Snippet: AA enhances COL1A1 biological activity and restores osteoblast function and COL1A1 regulation in the presence of Psl. A) ALP staining (red) of osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with (+AA), and in the Psl presence (+Psl) or absence (–Psl); Scale bar: 300 µm. B) Quantification of Mean Intensity of ALP (A.U.). C) Alzarin Red (AR) activity assay of osteo‐spheroids after 7 d with or without additional AA. Scale bar, 400 µm. D) Graph showing quantification of Mean Intensity of AR (A.U.). E) OsteoImaging mineralization assay quantifications of Mean Intensity (A.U.) of osteo‐spheroids incubated with or without additional AA for 7 d. F) Gene expression analysis of osteogenic markers ( BGLAP, DMP1, DLX3, RUNX2 ) of osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with (+AA), and in the Psl presence (+Psl) or absence (–Psl); Heatmap demonstrates the ∆CT averages. G) Gene expression analysis of COL1A1‐related genes ( PLOD1, PLOD3, DLX3, P3H1, P3H2, P3H3, LOX, SVCT2, COL1A2, COL22A1, IBSP, P4HA2, P4HA3, IFITM5 ) in osteo‐spheroids embedded in COL1A1 matrices, cultured for 7 d with or without ascorbic acid (AA), and in the presence (+Psl) or absence (–Psl) of prednisolone (Psl); Heatmap demonstrates the ∆CT averages H) Fold change of top collagen‐related putative metabolites elevated in the presence of endogenous ascorbic acid (+AA) within the 3D matrix of osteo‐spheroids after 7 d of Psl (+Psl) treatment, based on untargeted metabolomics analysis; fold change threshold > 2 with significance; FDR p‐value < 0.05. The data are expressed as mean ± SD; N = 3, n = 3; significant differences: * * p‐value < 0.01; *** p‐value < 0.005 ; **** p‐value < 0.0001.

Article Snippet: Human osteoblasts (HOBs) (PromoCell) were cultured in osteoblast growth medium (GM) (PromoCell) and differentiated in osteoblast mineralization medium (MM) (PromoCell).

Techniques: Activity Assay, Staining, Cell Culture, Mineralization Assay, Incubation, Gene Expression

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1) Product Images from "Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation"

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